Takara enzyme works with PCR-inhibiting agents
Takara enzyme works with PCR-inhibiting agents
TaKaRa Ex Taq DNA Polymerase is exceptionally robust, even in the presence of PCR inhibitors such as polyphenols found in crude DNA extracts from plant tissue. PrimeSTAR GXL DNA Polymerase is recommended when high fidelity is also required. Although PrimeSTAR GXL polymerase generally produces satisfactory results with the standard protocol, doubling the enzyme concentration may improve results if very high concentrations of inhibitors are present.
Alternatively, Terra PCR Direct Polymerase Mix allows direct amplification from crude samples that may contain high levels of PCR inhibitors. Terra PCR Direct polymerase can efficiently amplify a wide range of targets, including GC- and AT-rich targets. This enzyme can also be used for direct PCR from blood samples.
In cases where amplification products cannot be produced with these enzymes, it may be necessary to purify the template DNA.