Takara enzyme for AT-rich target sequences
Takara enzyme for AT-rich target sequences
TaKaRa Ex Taq DNA Polymerase and PrimeSTAR GXL DNA Polymerase are effective for amplifying AT-rich target sequences, such as genomic DNA containing introns or AT-rich mitochondrial DNA. We recommend PrimeSTAR GXL DNA Polymerase for amplifying AT-rich templates with high accuracy. In contrast to other PCR enzymes, PrimeSTAR GXL polymerase can amplify targets containing >60% AT content using a standard PCR protocol and the reaction buffer provided.
Note: PrimeSTAR GXL DNA Polymerase cannot be used to amplify bisulfite-treated DNA or other uracil-containing templates. For this application, try TaKaRa EpiTaq HS (for bisulfite-treated DNA).