Can RNA or cells stored in RNAlater be used as input for the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian?

RNAlater is NOT compatible with the SMARTer Stranded Total RNA-Seq Kit v3 – Pico cDNA synthesis, since it denatures proteins. RNA from cells stored in RNAlater must be purified prior to use as input for the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian cDNA synthesis.Stranded RNA-seq for mammalian [...]

Is the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with FFPE, PFA-fixed, or LCM cells?

No, the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is not compatible with cDNA synthesis directly from FFPE, PFA-fixed, or LCM cells, since such cells often contain since single-stranded DNA that can be randomly primed and used as a template by the reverse transcriptase, thus incorporating DNA in to the final SMARTer Stranded v3 Pico library.Stranded RNA-seq for mammalian [...]

Is the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with RNA purified from FFPE/PFA-fixed, or LCM cells?

The SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is compatible with purified RNA obtained from FFPE/PFA-fixed, and LCM cells, that does not contain any remnants of DNA, carrier or co-precipitant. RNA from FFPE or PFA-fixed cells must be decrosslinked and thoroughly treated with DNAse in order to remove all traces of DNA, since single-stranded DNA (often present in FFPE, [...]

Can I purchase R-Probes v3, ZapR v3, and ZapR Buffer (10X) separately?

No, R-Probes v3, ZapR v3, and ZapR Buffer (10X) are not available separately and are only included as components of the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian.Stranded RNA-seq for mammalian [...]

Can ZapR-mediated removal of rRNA sequences be used in other cDNA synthesis workflows or with pre-synthesized double-stranded cDNA?

No, ZapR-mediated removal of rRNA sequences has been designed to work exclusively as an integral part of the workflows of the SMARTer Stranded Total RNA-Seq Kit v2/ v3 - Pico Input Mammalian, SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian, and SMART-Seq Stranded kits.Stranded RNA-seq for mammalian [...]