Is the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with FFPE, PFA-fixed, or LCM cells?
Is the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian compatible with FFPE, PFA-fixed, or LCM cells?
No, the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is not compatible with cDNA synthesis directly from FFPE, PFA-fixed, or LCM cells, since such cells often contain since single-stranded DNA that can be randomly primed and used as a template by the reverse transcriptase, thus incorporating DNA in to the final SMARTer Stranded v3 Pico library.