What are the input requirements for the DNA SMART™ ChIP-Seq Kit?

DNA SMART ChIP-Seq Kit generates Illumina-compatible sequencing libraries from 100 pg–10 ng of singlestranded (ss) or double-stranded (ds) DNA resulting from chromatin immunoprecipitation (ChIP) experimentsChIP-seq and small RNA-seq [...]

Can the Guide-it SNP Screening Kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?

The current version of the Guide-it SNP Screening Kit can only be used to detect single-nucleotide substitutions, not longer insertions. We are, however, in the process of developing a new version of the kit that will enable detection of longer insertions using two Guide-it Flap Detectors in tandem. One detector yields a red fluorescent signal while the other one generates a green signal, [...]

Are there any limitations or special considerations regarding what target sequences can be analyzed with the Guide-it SNP Screening Kit?

The only design limitation one must consider when designing the probes is that the displacement oligo must not encode the sequences 5'-GGAGn-3' or 5'-GGAGNn-3' at its 3' terminus (where N can be any base, and n is the extra noncomplementary base that depends on the substitution being assayed). Displacement oligos containing these sequences will generate a background signal in the enzymatic [...]

How sensitive is the Guide-it SNP Screening Kit to the presence of mutations close to the site of the nucleotide substitution being assayed?

The Guide-it SNP Screening Kit is highly sensitive to the presence of mutations in the genomic target sequence surrounding the site of the substitution being assayed. The displacement oligo and the flap-probe oligo anneal to the region immediately adjacent to the SNP, and the presence of unknown mutations in that region would affect the annealing of the oligos with the PCR product and the [...]

How clean does the PCR of the genomic target sequence need to be for the Guide-it SNP Screening Kit?

The assay performs well regardless of whether extra PCR products are generated during amplification of the genomic target sequence. See the image below for example PCR amplification results that have been successfully used to detect nucleotide substitutions with this kit. Examples of PCR-amplified target DNAs used as samples with the Guide-it SNP Screening Kit. PCR products visible in each gel [...]