Do I have to dilute the NTI buffer during purification of the SMARTer smRNA PCR reaction mix?
Do I have to dilute the NTI buffer during purification of the SMARTer smRNA PCR reaction mix?
Follow the standard NucleoSpin PCR Clean-Up and Gel Extraction User Manual protocol without diluting the NTI buffer: 200 ul NTI Buffer is added to 100ul PCR reaction mix.