What's In-Fusion compatibility with small inserts?
Seamless cloning is not suitable for small inserts of less than 50 bp. This is because of the exonuclease activity removing a 15–30 bp region at both ends, which may affect a significant percentage of the insert sequence.
We recommend using a traditional, ligase-based cloning method for inserts smaller than 50 bp.
2560 Orchard Parkway
San Jose, CA 95110
Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.