In an In-Fusion Cloning reaction, how many colonies should I expect from the negative control?
In an In-Fusion Cloning reaction, how many colonies should I expect from the negative control?
The negative control provided with the kit typically produces fewer than 5% blue colonies; the number of white colonies produced varies slightly depending on the strain. In general, fewer than 5% of the white colonies on an experimental plate contain background. It has been our observation that ?95% of the colonies on experimental plates are correct. This speaks to In-Fusion technology's high level of cloning efficiency, i.e., the percentage of correct colonies recovered regardless of the total number of transformed colonies present.