What are the compatible vectors for In-Fusion?
What are the compatible vectors for In-Fusion?
Any linear vector can be used for In-Fusion Cloning, regardless of size, composition, or available restriction site(s). The cloning reaction is followed by the rescue of a circular recombinant construct in E. coli, but please note that circular vectors are not compatible with the cloning reaction itself. Additionally, In-Fusion Cloning does not allow the assembly of covalently linked linear DNA molecules.