Takara Bio Support Portal

Search FAQs

basic

advanced

Exact phrase:

  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • Sign in
  • Sign up
  • Home
  • Categories
    Nucleic Acid Purification (Macherey Nagel)
    NGS
    PCR
    In-Fusion
    qPCR
    -- All Categories --
  • |
  • Submit new ticket
  • Sign in
  • Sign up
  1. Home
  2. Nucleic Acid Purification (Macherey Nagel)
  3. Can I use the NucleoProtect reagent for collecting nasal swabs?

Can I use the NucleoProtect reagent for collecting nasal swabs?

  • Date updated 2023-06-27
  • Categories
  • Nucleic Acid Purification (Macherey Nagel)
  • Tags
  • ProdCompatibility
  • ProdSampletype

Can I use the NucleoProtect reagent for collecting nasal swabs?

No. Most swabs retain too much of the RNA protect solution and this will interfere with RNA isolation.
Therefore we don't recommend using the NucleoProtect reagent for swabs.

Nucleic acid purification products

related articles

  • Is it OK to freeze cells in the MR1+TCEP at -80 °C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
    • 365
    • 0
  • Can customer use samples lysed in TriZol on the NucleoSpin® RNA Set for NucleoZOL™?
    • 262
    • 0
  • Can you proceed with NucleoSpin RNA XS if your samples are in Trizol?
    • 305
    • 0
  • What is Carrier RNA is made of?
    • 482
    • 0
  • Can I store samples in the RA1 lysis buffer of NucleoSpin RNA kit?
    • 305
    • 0
  • What kit do you recommend for isolating RNA from OCT-fixed tissue blocks?
    • 284
    • 0
  • Do you have any special recommendations for isolating viral RNA from breast milk?
    • 302
    • 0
  • Can I purchase RCU buffer from 740910 NucleoSpin RNA Clean-up Maxi?
    • 292
    • 0
  • Can I use the NucleoProtect reagent for collecting nasal swabs?
Rate this article
Subscribe to alerts for this article below
Your email is now subscribed to alerts for this article
Your email is not subscribed to alerts for this article
Unsubscribe to alerts for this article below

  • Nucleic Acid Purification (Macherey Nagel)22 Articles

    • Are NucleoMag Beads Silica-based?
    • Can I purchase RCU buffer from 740910 NucleoSpin RNA Clean-up Maxi?
    • What are the dimensions of the NucleoBond® Xtra 96 Plates EF?
    • Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.
    • Do you have any special recommendations for isolating viral RNA from breast milk?
    • Can I use the NucleoProtect reagent for collecting nasal swabs?
    • What kit do you recommend for isolating RNA from OCT-fixed tissue blocks?
    • Can I store samples in the RA1 lysis buffer of NucleoSpin RNA kit?
    • What is Carrier RNA is made of?
    • Can you proceed with NucleoSpin RNA XS if your samples are in Trizol?
    • What are the sizes of holes of the NucleoBond Smart Rack?
    • What are the differences between 744950 (KingFisher 96 Accessory Kit A) and 744951 (KingFisher 96 Accessory Kit)?
    • Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
    • Can customer use samples lysed in TriZol on the NucleoSpin® RNA Set for NucleoZOL™?
    • How do I select NucleoMag® Beads for my specific application?
    • What are the dimensions of the NucleoSpin® 96 Plasmid Filter Plates?
    • Is it OK to freeze cells in the MR1+TCEP at -80 °C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
    • Is NucleoZOL compatible with NucleoMag kits (Pathogen)?
    • What is the diameter of the NucleoMag Magnetic bead particles? 
    • What are the dimensions of the NucleoVac™ 96 Vacuum Manifold?
    • Can the NucleoSpin Plasmid Purification columns substitute the NucleoSpin Plasmid Transfection- grade columns to isolate Endo-free plasmid DNA?
    • Can I use the NucleoSpin Gel & PCR Clean-up kit for removing the SYBR Green Dye?
  • NGS0 Articles

    • NGS-RNA2 Articles
      • What rRNA does RiboGone™ - Mammalian remove?
      • What RNA quality is needed for performing full length Next Generation Sequencing (NGS)?
    • RNA-seq general64 Articles
      • What are the most common artifacts of cDNA synthesis with SMARTer kits?
      • Why is quantification of NGS libraries by qPCR better than using other methods?
      • What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?
      • Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?
      • Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?
      • How do you explain the presence of introns in the final SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian cDNA library?
      • What are the nucleotide sequences of the adapters required for trimming prior to the software analysis of the sequencing reads?
      • Will collected cells change the volume of the cell collection buffer?
      • How should cells be collected for cDNA synthesis?
      • What are abnormal Bioanalyzer traces for SMARTer stranded kits?
      • What are abnormal Bioanalyzer traces for SMARTer Ultra low kits?
      • What are abnormal Bioanalyzer traces for all SMARTer kits?
      • Can I substitute alternative products for any of the recommended additional materials?
      • How can I ensure efficient cDNA purification using SPRI beads?
      • Why do I have to use different magnetic devices for SPRI bead purification of cDNA?
      • Why do I have to perform a negative control during SMARTer cDNA synthesis?
      • Why should I perform a positive-control cDNA synthesis reaction?
      • Why do library concentrations obtained with a qPCR-based method differ from those obtained by other methods?
      • Why is quantification of NGS libraries by qPCR better than using other methods?
      • Why should I quantify my libraries prior to sequencing?
      • How should sequencing libraries be prepared using SMARTer stranded kits?
      • What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?
      • Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?" "
      • Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?
      • How do I pool cDNA libraries generated with the Low Input Library Prep kits for Illumina sequencing?
      • What is the expected size range of Covaris-sheared cDNA after library preparation?
      • What are the advantages of library prep with the ThruPLEX DNA-Seq Kit?
      • How should I set up the Peak Incident Power (W) for the Covaris S220 system?
      • What type of Covaris machine did you use to optimize the shearing parameters?
      • What is the expected size range of Covaris-sheared double-stranded (ds) cDNA?
      • What method should I use to prepare cDNA generated with SMARTer Ultra low kits for sequencing?
      • How should sequencing libraries be prepared from cDNA generated with SMARTer Ultra low kits?
      • Can I analyze unpurified double-stranded (ds) cDNA for PCR cycle optimization?
      • What can I do if I have low cDNA yield?
      • How do I determine the double-stranded (ds) cDNA yield
      • What is the expected double-stranded (ds) cDNA yield?
      • What is the expected size distribution of double-stranded cDNA generated by SMARTer cDNA synthesis kits?
      • How do I analyze double-stranded (ds) cDNA quality?
      • Can I collect cells directly in Reaction Buffer?
      • Can I freeze collected cells prior to cDNA synthesis?
      • What is the recommended volume of Reaction Buffer for various amounts of cells when using SMARTer Ultra Low kits?
      • How do I lyse cells for direct cDNA synthesis?
      • What media have been tested for compatibility with direct cDNA synthesis from intact mammalian cells?
      • Can I use more than 1,000 cells as input for direct cDNA synthesis using SMARTer Ultra low kits?
      • How many cells can I use for direct cDNA synthesis with SMARTer Ultra low kits?
      • Which SMARTer Ultra low kit is recommended for direct cDNA synthesis from whole mammalian cells?
      • What are general considerations for cDNA synthesis directly from cells?
      • How do I shear rRNA-depleted RNA?
      • What ribosomal RNA depletion methods do you recommend?
      • Why do I need to remove ribosomal RNA?
      • Which RNA purification kits are compatible with the SMARTer Universal kit?
      • SMARTer Ultra low kits: what RNA purification kits are recommended?
      • What methods can I use to assess RNA quality and quantity?
      • What are the requirements for RNA quality and quantity when using SMARTer Ultra Low kits?
      • What are RNA sample preparation requiremtns for SMARTer Ultra low & SMARTer universal kits
      • What SMARTer cDNA synthesis kits are compatible with Ion Torrent sequencing platforms?
      • Can I use SMARTer kits for the analysis of mature miRNAs?
      • What kit is recommended for degraded total RNA samples?
      • What kit is recommended for full-length or degraded total RNA with strand information maintained?
      • I am currently using a legacy SMARTer Ultra low kit, can I switch to the SMART-Seq v4 kit?
      • What are the benefits of using the fourth-generation SMART-Seq v4 kit versus other ultra-low input mRNA-seq kits?
      • What kit is recommended for whole cells or ultra-low input total RNA samples: SMARTer Ultra low kits (dT-primed)
      • What kit is recommended for whole single cells: SMART-Seq Single Cell Kit (full-length mRNA-seq)
      • Which kit is right for my application?
    • Indexing15 Articles
      • What are the index adapter sequences after preparation using ThruPLEX DNA libraries?
      • For indexes in 96-well-plate format: can I generate just a few samples at a time?
      • Are the Takara Bio DNA unique dual indexes the same as the Illumina TruSeq UD or IDT for Illumina UD sequences?
      • Can libraries made with your DNA unique dual index kits be pooled with libraries made using TruSeq CD indexes?
      • Are indexing kits from other companies compatible with ThruPLEX and PicoPLEX library prep kits?
      • Are Takara Bio indexing kits compatible with library prep kits from other companies?
      • What guidelines should I follow when pooling indexes?
      • What should I consider when multiplexing samples?
      • Can I perform single-read runs and still obtain both index sequences?
      • Can I do paired-end sequencing with single-indexed libraries?
      • What is the difference between single and paired-end reads?
      • How can I mitigate index crosstalk or ""sample bleeding"" issues?
      • What is index hopping?
      • What are the advantages of using dual- versus single-indexed libraries?
      • How does dual indexing work?
  • PCR50 Articles

    • Takara enzyme for multiplex PCR
    • Takara Direct amplification from tissue
    • Takara enzyme for highest sensitivity
    • Takara enzyme for colony PCR
    • Takara solution for gel electrophoresis after PCR (e.g., genotyping screens)
    • Takara enzyme for Paraffin/FFPE sections
    • Takara enzyme works with PCR-inhibiting agents
    • Takara enzyme for bisulfite-treated DNA
    • Takara enzyme for AT-rich target sequences
    • Takara enzyme for Fast PCR
    • Takara enzyme for Long-range PCR
    • Takara enzyme for GC-rich target sequences
    • Takara enzyme with highest fidelity
    • What precautions should be taken when using inosine-containing primers?
    • PrimeSTAR Max and PrimeSTAR GXL DNA polymerases have very high fidelity; how was fidelity measured for these enzymes?
    • How can I compare error rates of different high-fidelity polymerases?
    • What is meant by polymerase fidelity? What applications require a high-fidelity polymerase?
    • Which polymerases generate blunt ends versus A-overhangs?
    • What is touchdown PCR (TD-PCR) and when would I need to use it?
    • What is nested PCR?
    • What factors are critical for multiplex PCR?
    • What are PCR artifacts?
    • What factors contribute to PCR-introduced mutations?
    • What types of mutations can be caused by PCR?
    • What is PCR overcycling? How do I know if my product is overcycled?
    • What are PCR inhibitors?
    • What can I do if the PCR generates errors?
    • How can I decontaminate if I have PCR contamination?
    • What are some sources of PCR contamination?
    • If PCR generates a smear after running the products on a gel, what can be done to improve the results?
    • If there are nonspecific amplification bands, what can be done to improve specificity?
    • If no amplification products are obtained, what parameters should be considered first when troubleshooting?
    • What is the role of salt in PCR reactions?
    • What is the role of magnesium in PCR, and what is the optimal concentration?
    • How can I optimize PCR conditions for AT-rich templates?
    • Can DMSO be added to improve amplification of GC-rich templates?
    • What are the critical factors for amplification of GC-rich templates?
    • What are the critical factors for amplification of long genomic targets?
    • What is the optimal amount of DNA template that should be used for PCR?
    • Which extension temperature should I use, 68°C or 72°C?
    • Should I use a three-step or a two-step PCR protocol?
    • When optimizing PCR conditions, which conditions are particularly important?
    • Are there any special considerations when handling PCR enzymes?
    • How should lyophilized PCR premixes be stored?
    • How are PCR polymerases shipped, and how should they be stored and handled after receipt?
    • What precautions should be taken when using inosine-containing primers?
    • How should oligos be purified for PCR?
    • What primer concentration should be used for PCR?
    • How do I calculate the melting temperature (Tm) of primers?
    • How do I determine if a template is GC rich?
  • In-Fusion86 Articles

    • How does In-Fusion Cloning work?
    • What is the efficiency of In-Fusion Cloning?
    • What is In-Fusion Cloning?
    • Can In-Fusion be used to clone a microRNA (miRNA) precursor
    • Can In-Fusion be used to clone shRNA (small hairpin RNA)?
    • What's In-Fusion's compatibility with site-directed mutagenesis?
    • What's In-Fusion's compatibility with multiple-fragment cloning?
    • What's In-Fusion's compatibility with large inserts?
    • What's In-Fusion's compatibility with large vectors?
    • How to clone a gene of interest in-frame with a fluorescent protein (alternative protocol)
    • What are the vector and insert properties related to transformation efficiency
    • What is the optimal transformation amounts for In-Fusion?
    • What are the bacterial strains not recommended for In-Fusion Cloning?
    • What is the bacterial cell competency required for In-Fusion?
    • What are the PCR requirements for In-Fusion reaction?
    • What are the tips for amplification with In-Fusion PCR primers?
    • What are the compatible polymerases for In-Fusion?
    • What’s the optimal length of homologous overlaps?
    • What are the considerations for the location of homologous overlaps?
    • What's the optimal incubation time for In-Fusion?
    • How should I set up control reactions for In-Fusion?
    • What's the molar ratios between vector and insert for In-Fusion?
    • Is In-Fusion capable of working with site-directed mutagenesis?
    • How do I split the 15-nt homologous overlap among adjacent DNA fragments?
    • Can I use In-Fusion to insert external nucleotide sequences?
    • Can I modify translational reading frames during In-Fusion?
    • How do I preserve restriction-site using In-Fusion?
    • What are the features of Takara online primer design tools?
    • How should I generate homologous overlaps of DNA fragments?
    • What are PCR primer design considerations for compatible with In-Fusion Cloning?
    • What's In-Fusion compatibility with multiple inserts?
    • What's In-Fusion compatibility with small inserts?
    • What's In-Fusion compatibility with large inserts?
    • What insert sources are compatible with In-Fusion?
    • What are vector linearization and purification options for In-Fusion?
    • What's the allowed vector size for In-Fusion?
    • What are the compatible vectors for In-Fusion?
    • How should I plan my In-Fusion experiment
    • How should I plan my In-Fusion experiment
    • How can I ensure transformation efficiency and overall cloning efficiency?
    • Can I use electroporation to transform the In-Fusion Cloning reaction mix?
    • In an In-Fusion Cloning reaction, how many colonies should I expect from the negative control?
    • Can I transform In-Fusion Cloning reaction mixtures in amounts larger than what is recommended in the user manual?
    • What bacterial strains are compatible with In-Fusion Cloning?
    • Can I use TOP10 cells for In-Fusion Cloning?
    • Will cloning efficiency increase if I use a longer incubation time for the In-Fusion Cloning reaction?
    • Can I modify the length of the homologous overlap? Will a longer overlap improve In-Fusion Cloning efficiency?
    • What are the recommended insert-to-vector molar ratios for In-Fusion Cloning?
    • Can I use In-Fusion Cloning to clone GC-rich DNA fragments?
    • Can I clone an oligonucleotide/shRNA oligonucleotide using In-Fusion Cloning?
    • Can I use In-Fusion Cloning for mutagenesis?
    • Will In-Fusion technology allow cloning of an insert if the sites of complementarity are located at a distance from the linearized vector termini?
    • Can I use In-Fusion Cloning to clone a DNA fragment generated by restriction digest?
    • Can I use a circular cloning vector for In-Fusion Cloning?
    • Can I use In-Fusion Cloning to assemble a covalently linked linear DNA molecule?
    • Is In-Fusion Cloning compatible with vectors carrying repeated sequences?
    • Are large cloning vectors compatible with In-Fusion Cloning?
    • Do I have to dephosphorylate the termini of a linearized vector for In-Fusion Cloning?
    • Does In-Fusion Cloning preserve the restriction site(s) used to linearize the vector?
    • What cloning vectors are compatible with In-Fusion Cloning?
    • What is the smallest DNA fragment compatible with In-Fusion Cloning?
    • What is the largest DNA fragment compatible with In-Fusion Cloning?
    • Do I have to purify the PCR-amplified insert and/or vector prior to performing the In-Fusion Cloning reaction?
    • Can I split the homologous 15-nt overlap between the insert and vector, or adjacent inserts?
    • Can I clone multiple fragments into one vector in a single In-Fusion Cloning reaction?
    • Do PCR-generated 3' A-overhangs interfere with In-Fusion Cloning?
    • What PCR polymerases are recommended for amplification of the In-Fusion cloning insert?
    • What oligonucleotide quality is required for an In-Fusion PCR primer?
    • Do I need to use phosphorylated PCR primers for In-Fusion Cloning?
    • For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?
    • Can small external sequences be included in the In-Fusion PCR primer?
    • How do I clone my gene of interest in the same translational reading frame as a tag present in the cloning vector (e.g., fluorescent protein, Myc, HA, etc.)?
    • How can I alter the reading frame when performing In-Fusion Cloning?
    • How do I calculate the 15-nt overlap if the vector is linearized via restriction digest, generating a 5' or 3' overhang?
    • Why are homologous overlaps important for In-Fusion Cloning reactions?
    • What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?
    • What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?
    • How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?
    • How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?
    • What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?
    • How does In-Fusion Snap Assembly compare to NEBuilder HiFi DNA Assembly?
    • In-Fusion HD and In-Fusion Snap Assembly bundles include different PCR polymerases. Should I expect a difference in performance?
    • What is the difference between In-Fusion Snap Assembly and In-Fusion HD Cloning?
    • Does the In-Fusion Cloning method introduce errors into the sequence?
    • What is Cloning Enhancer (CE)?
    • What is the difference between In-Fusion Snap Assembly and In-Fusion Snap Assembly EcoDry?
  • qPCR24 Articles

    • What is the storage temperature of Premix Ex Taq™ (Probe qPCR)?
    • What is the storage temperature of TB Green® Premix Ex Taq™ (Tli RNase H Plus)?
    • What is the difference between RR390A (Premix Ex Taq™ (Probe qPCR)) and RR039A (Premix Ex Taq™ DNA Polymerase (Perfect Real Time) kits?
    • What is the role of Tli RNase H in qPCR reaction?
    • Which SYBR Premix should I choose – RR420 or RR820? What is the difference between these premixes?
    • Can you mix the ROX™ reference dyes and TB Green® Premix Ex Taq™ to help avoid pipetting errors?
    • What is the composition of TB Green® Premix Ex Taq™?
    • Why is the amplicon length so limited in qPCR?
    • What PCR product size is optimal for real-time PCR?
    • How much ROX is needed for each instrument? Why are the ROX concentrations different?
    • What is ROX used for?
    • Should I choose Probe or Dye Based chemistry for my qPCR experiments?
    • What are the differences between absolute and relative quantification?
    • I understand that there are two ways to determine the Ct value. What are they?
    • What is the sensitivity of qPCR?
    • What is the appropriate number of replicates (n)?
    • What method(s) should be used for analyzing qPCR amplification products?
    • What standard samples are recommended for preparing calibration curves?
    • Target gene expression levels are often normalized to the expression of a “housekeeping gene” (reference gene) to correct for differences in the amount of input RNA and variations in reaction efficiency. How do I select a suitable housekeeping gene?
    • How can amplification of genomic DNA in total RNA samples be avoided?
    • Which is a better starting sample for preparing RT-qPCR calibration curves, RNA or cDNA?
    • What are the benefits and application of two-step RT-qPCR?
    • What are the benefits and application of one-step RT-qPCR?
    • Comparison between Takara RT-qPCR kits.
  • Protein Science0 Articles

    • Protein Expression1 Articles
      • What's the copy number of the pBE-S vector from B. Subtilis Secretory Protein Expression System?
    • Protein Purification1 Articles
      • Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery
  • Stem Cells93 Articles

    • How does reprogramming method affect the application of DEF-CS system?
    • What are the candidates for substitute pluripotency tests?
    • What are the substitute convenient tests for pluripotency?
    • What are the reliable tests for differentiation?
    • What are the reliable tests for proof of pluripotency?
    • What is the survival rate of single cell cloning in the DEF-CS system
    • What are the common pluripotency markers?
    • Does cell morphology change with different culturing systems?
    • Can DEF-CS be used to differentiate cells if additional factors are added?
    • Do you have any data/experience with using DEF-CS media on Matrigel?
    • How long can single cells be cultured in DEF-CS? What's the upper limit of passages caan stem cells survive in DEF-CS without affecting karyotype ?
    • what's COAT-1?
    • Tips for single cell cloning.
    • Does DEF-CS contain ROCK inhibitors?
    • Any useful tips for transitioning/thawing cell culture in DEF-CS?
    • Is this possible and what is the advantage to growing iPS cells in suspension?
    • What's the difference between Laminin-511 and Laminin-521
    • What is LIF?
    • Why does DEF-CS 500 Xeno-Free Culture Medium product contain 2 additives, while the standard DEF-CS 500 Culture System product contains 3? Are any of the additives the same between the 2 products?
    • How to transfer iPS cells to DEF-CS
    • What is the freeze/thaw stability of the DEF-CS 500 Basal Media and Additives (can they undergo multiple freeze thaw cycles)?
    • Can DEF-CS cultured iPS cells be differentiated to all 3 germ layer cells?
    • Can cells grown in DEF-CS be differentiated into neurons?
    • What's Y-27632?
    • What's NS21 supplement?
    • What's a common medium for neuron culture?
    • What's in B18 supplement?
    • Can the hiPS-derived hepatocytes be dissociated and frozen?
    • Can the hiPS cell-derived hepatocytes be dissociated and reseeded?
    • Can the DE cells be dissociated and frozen?
    • Can the DE cells be dissociated and reseeded?
    • Can I perform the hiPS cell hepatocyte differentiation in 3D?
    • Can DEF-CS cultured iPS cells be differentiated to all 3 germ layer cells?
    • Can I count the cells during hiPS cell-derived hepatocyte differentiation using an automated cell counter?
    • How much do small deviations from the protocol (e.g., a daily medium change delayed 12–18 hours, etc.) affect the functionality of the hiPS cell-derived hepatocytes? Which step(s) are the most critical ones?
    • Which culture vessels are suitable?
    • Is it possible to expand the hiPS cell-derived hepatocytes?
    • For how many passages can the DE cells be maintained? Can the DE cells be scaled up?
    • How is the Cellartis iPS Cell to Hepatocyte Differentiation System shipped?
    • Can CYP enzyme activities be induced in hepatocytes derived with the Cellartis iPS Cell to Hepatocyte Differentiation System?
    • Do hiPS cell-derived hepatocytes that are generated with this system show formation of bile canaliculi?
    • Do hiPS cell-derived hepatocytes exhibit drug metabolism activity?
    • On what day can I start using the hepatocytes?
    • How do the hepatocytes derived with this system compare to human primary hepatocytes?
    • History of Laminin
    • How were CYP enzyme activities measured?
    • Do hiPS cell-derived hepatocytes that are generated with this system express adult hepatocyte markers?
    • How many hiPS cell-derived hepatocytes can I expect to get from one kit?
    • What features can I expect from the hepatocytes derived with the Cellartis iPS Cell to Hepatocyte Differentiation System?
    • What happens to the hPS cell-derived hepatocytes after Day 32? Do they lose activity, no longer look like hepatocytes, or die?
    • Can the differentiation kits be used for differentiation of pluripotent stem cells from other species than human?
    • Which cell types make up the other 6% that are HNF4?-negative in the hiPS cell-derived hepatocytes?
    • How pure can I expect the final population of hPS-derived hepatocytes to be?
    • How pure can I expect the final population of definitive endoderm cells to be?
    • If I have the Cellartis iPS Cell to Hepatocyte Differentiation System, do I need to purchase DEF-CS culture medium separately?
    • What kind of pluripotent stem cells can I start with?
    • How is the Cellartis iPS Cell to Hepatocyte Differentiation System different from the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System combined with the Cellartis Hepatocyte Differentiation Kit?
    • Does the DEF-CS culture system allow automation?
    • How many passages can be performed on human iPS cells cultured with the DEF-CS system?
    • How frequently should I passage cells grown in DEF-CS
    • How often should I change the medium for cells grown in DEF-CS?
    • How many cells can be grown with the DEF-CS system?
    • What is the expected growth rate of the cells cultured in DEF-CS?
    • Why doesn't the provided volume of DEF-CS GF-3 correspond to the provided volume of DEF-CS Basal Medium?
    • Does the DEF-CS basal medium contain antibiotics?
    • What enzyme is used to passage cells cultured in DEF-CS?
    • What is the composition of the DEF-CS medium?
    • What is included in the DEF-CS system?
    • What are the characteristics of human iPS cells cultured with the DEF-CS system?
    • Can you culture Cellartis enhanced hiPS-HEP cells as 3D spheroids?
    • I observe dome-like structures in Cellartis enhanced hiPS-HEP cell culture. Is this normal?
    • I observe droplets in Cellartis enhanced hiPS-HEP cell culture. Is this normal?
    • How were CYP enzyme activities measured in Cellartis enhanced hiPS-HEP cells
    • Is it possible to proliferate Cellartis enhanced hiPS-HEP cells?
    • Can Cellartis enhanced hiPS-HEP cells go without changing media over the weekend?
    • Why should partial media changes be performed from Day 3 onward?
    • Why do the cells need to be washed on Day 1 after plating?
    • Does Cellartis Enhanced hiPS-HEP Long-Term Maintenance Medium contain dexamethasone, hydrocortisone, and DMSO?
    • Can other maintenance media or coatings be used for culturing Cellartis enhanced hiPS-HEP cells?
    • Which culture vessels are suitable for Cellartis enhanced hiPS-HEP cells?
    • How are Cellartis enhanced hiPS-HEP cells shipped?
    • Do Cellartis enhanced hiPS-HEP cells show formation of bile canaliculi?
    • How do we know Cellartis enhanced hiPS-HEP cells are mature hepatocytes?
    • Cellartis enhanced hiPS-HEP cells are >90% pure. Which cell types make up the other 10%?
    • Does fetal CYP3A7 contribute to overall CYP activity in these cells?
    • Can CYP enzyme activities be induced in Cellartis enhanced hiPS-HEP cells?
    • Do Cellartis enhanced hiPS-HEP cells express phase II enzymes?
    • Do Cellartis enhanced hiPS-HEP cells express transporter proteins?
    • How soon after thawing Cellartis enhanced hiPS-HEP cells can I begin testing?
    • What is the difference between a Cellartis enhanced hiPS-HEP v2 kit and the older version?
    • Are Cellartis enhanced hiPS-HEP cells available from several hiPS cell lines?
    • Are Cellartis enhanced hiPS-HEP cells genetically engineered?
    • What is the source of Cellartis enhanced hiPS-HEP cells?
  • Genome Editing63 Articles

    • Can the Guide-it SNP Screening Kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?
    • Are there any limitations or special considerations regarding what target sequences can be analyzed with the Guide-it SNP Screening Kit?
    • How sensitive is the Guide-it SNP Screening Kit to the presence of mutations close to the site of the nucleotide substitution being assayed?
    • How clean does the PCR of the genomic target sequence need to be for the Guide-it SNP Screening Kit?
    • Can the Guide-it SNP Screening assay be used to distinguish homozygotes from heterozygotes?
    • Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?
    • What cell densities are suitable for analysis with the Guide-it SNP Screening Kit?
    • Which cell types have been analyzed using the Guide-it SNP Screening Kit? Can the kit be used for suspension cells?
    • Do I need to use column-purified genomic DNA for the Guide-it SNP Screening Kit?
    • Can the Guide-it SNP Screening Kit be used to detect successful homologous recombination (HR) in edited populations?
    • Can the Guide-it SNP Screening Kit be used for genotyping?
    • Can I use the kit if I have no idea what the substitution is?
    • Does the Guide-it SNP Screening Kit allow for detection of any nucleotide substitution regardless of the genomic target site?
    • Can a qPCR thermal cycler be used for fluorescence detection step of Guide-it SNP Screening Kit?
    • What do I need for the detection of the assay signal?
    • What is included in the Guide-it SNP Screening Kit?
    • How does Guide-it Flapase detect the edited nucleotide?
    • What oligos do I need to order to perform the assay?
    • What is the workflow for the Guide-it SNP Screening Kit?
    • How can I increase the frequencies of edited clones with only one modified allele and the other one encoding the wild-type sequence?
    • What are additional methods for improving HDR efficiency?
    • What are the effects of chromatin configuration on HDR efficiency?
    • Does the HDR insertion site need to be directly next to the PAM?
    • Is it helpful to use HDR templates that introduce silent mutations in the PAM sequence or sgRNA seeding region?
    • What are optimal lengths for HDR template homology arms?
    • With ssODN or ssDNA, is it advantageous to use HDR templates that are homologous to the sense or antisense strand at the genomic target region?
    • How do ssODNs differ from ssDNA? When is it better to use one vs. the other?
    • What are the advantages and disadvantages of using single-stranded vs. double-stranded HDR templates for engineering gene knockins?
    • How do I design my HDR template?
    • What is HDR for Gene Editing?
    • What additional kits are needed for preparing NGS libraries downstream of screening?
    • What is the advantage of having an mCherry reporter gene in the sgRNA vector?
    • Where can I find all the vector maps for the Guide-it CRISPR Genome-Wide sgRNA Library System?
    • What are the advantages of your lentiviral packaging system, and how is it different from other libraries?
    • Does every sgRNA in the library work?
    • Why is guide RNA scaffold design an important feature of the library?
    • What is the Brunello algorithm that was used to create the library?
    • Why are there four sgRNAs per gene and not eight?
    • Why are there 76,612 different guides in the Guide-it CRISPR Genome-Wide sgRNA Library?
    • Which genes are targeted using your library? Where can I find a list of the targeted genes?
    • What is the design of the vector backbones used in your library?
    • What are the advantages of using an sgRNA library in Takara Bio’s Lenti-X Single Shots format vs. starting from plasmid or lentiviral particles?
    • What are the kit components of the Guide-it CRISPR Genome-Wide sgRNA Library System?
    • The Guide-it library is supplied in a lyophilized Lenti-X Single Shots format. What does that mean?
    • Why should I buy the Guide-it CRISPR Genome-Wide sgRNA Library System?
    • What is a typical timeline for a genome-wide CRISPR screen using a pooled lentiviral sgRNA library?
    • Is there any screening data for the Guide-it CRISPR Genome-Wide sgRNA Library System?
    • Should I be worried about false positives or off-target effects when screening using the Guide-it CRISPR Genome-Wide sgRNA Library System?
    • How much DNA do I need to isolate prior to performing NGS?
    • Are the guide RNAs evenly represented in your library? Does this change when producing and transducing virus?
    • Why do you recommend a 30–40% transduction efficiency and not higher?
    • Why is the expression level of Cas9 important?
    • How many total transduced cells do I need for a whole genome sgRNA-library screen and why?
    • Which cells should I use for genome-wide sgRNA screen?
    • Why do I need to use next-generation sequencing (NGS) to analyze the results of the screen? How can I perform this analysis?
    • Why do we need multiple guides targeting the same gene?
    • Why are guide RNA libraries supplied on lentivirus vectors but not on regular plasmids?
    • What are positive and negative CRISPR library screens?
    • Pooled sgRNA libraries vs. pooled RNAi libraries vs. targeted arrayed sgRNA libraries
    • What are some example applications of pooled whole-genome sgRNA library screens?
    • Where can I find more information about CRISPR/Cas9-mediated gene knockout?
    • How do I perform a genome-wide CRISPR knockout screen?
    • Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery
  • Viral Delivery13 Articles

    • Adenovirus10 Articles
      • What is PFU?
      • What types of adenoviruses are compatible with the Adeno-X Rapid Titer Kit?
      • Can I use adenovirus purified with Adeno-X purification kits for in vivo studies?
      • Can I purify adenovirus from cell supernatants or cells combined with supernatant using Adeno-X purification kits?
      • What types of adenoviruses are compatible with Adeno-X purification kits?
      • Do you have adenoviral vectors for tet-inducible gene expression?
      • What type of adenovirus are Takara Bio’s adenoviral vectors derived from?
      • What is the backbone of Takara Bio’s adenoviral vectors?
      • What are the cloning/packaging capacities of Takara Bio's adenoviral vectors?
      • What is Adeno-X Adenoviral System 3?
    • Retrovirus18 Articles
      • What are CFU?
      • What are IFU?
      • What is MOI?
      • Can I use the Retro-X qRT-PCR Titration Kit to titer MSCV-based retroviruses?
      • How does the viral envelope affect the Retro-X Concentrator’s efficiency?
      • Can I use the Retro-X Concentrator to concentrate a lentivirus?
      • What are self-inactivating (SIN) retroviral vectors (Retro-X Q Vectors)?
      • Can I use a retroviral vector to perform regular plasmid transfections?
      • Can primary cells be infected with a retrovirus?
      • Which retroviral packaging cell line or system should I use to transduce my target cells?
      • Are Takara Bio’s retroviral packaging cell lines and retroviral systems compatible with retroviral vectors from other sources?
      • Are Takara Bio’s retroviral vectors compatible with retroviral packaging systems from other sources?
      • How do you guarantee the biosafety of Takara Bio’s retroviral packaging cell lines?
      • Are there size limitations for cloning into IRES vectors?
      • How do bicistronic (IRES) vectors express two proteins simultaneously?
      • Do I have to include an internal poly(A) signal in the retroviral expression cassette for my gene of interest (GOI)?
      • What fraction of the wild-type MMLV genome is present in Takara Bio’s retroviral vectors?
      • What is the cloning/packaging capacity of a retroviral vector from Takara Bio?
    • AAV32 Articles
      • FAQ
      • What are the main features of adeno-associated viruses?
      • I want to purify my AAV vector. Which kit do you recommend?
      • What are the advantages of the AAVpro Helper Free System in comparison with AAV production kits from other companies?
      • Do you offer AAV control vectors?
      • Infection of cultured cells cannot be confirmed; what could be the cause?
      • What cells are suitable for the production of AAV particles?
      • What is “helper-free” production?
      • How much AAV should be used for general animal experiments?
      • What precautions should be taken when working with recombinant AAV?
      • What are the features of different AAV serotypes?
      • What features of AAV makes the virus a good vector for gene delivery?
      • Upon addition of AAV Extraction Solution B, the solution turned pink; is this a problem?
      • Is AAVpro Extraction Solution compatible with any AAV serotype?
      • What are the advantages of the AAVpro kit in comparison to other conventional titration methods?
      • Which serotypes can be measured with the AAVpro Titration Kit (for Real Time PCR) Ver.2?
      • What is standard yield of AAV particles using the AAVpro Purification Kit (All Serotypes)?
      • For administration to animals, how should AAV particles be purified?
      • What number of cells can be used for AAV purification with the AAVpro Purification Kit (All Serotypes)?
      • What are the benefits of using the AAVpro Purification Kit (All Serotypes)?
      • I want to purify my AAV vector. Which kit do you recommend?
      • What are the advantages of the AAVpro Helper Free System in comparison with AAV production kits from other companies?
      • Do you offer AAV control vectors?
      • Infection of cultured cells cannot be confirmed; what could be the cause?
      • What cells are suitable for the production of AAV particles?
      • What is “helper-free” AAV production?
      • How much AAV should be used for general animal experiments?
      • Are there published examples of AAV2 being used for in vivo gene transfer?
      • What precautions should be taken when working with recombinant AAV?
      • What are the features of different AAV serotypes?
      • What features of AAV makes the virus a good vector for gene delivery?
      • What are the main features of adeno-associated viruses?
    • Lentivirus29 Articles
      • How accurately do GV titers correlate to infectious units (IFU)?
      • What is a GoStix Value (GV) titer?
      • What is p24 in Lentiviral titration kits?
      • What are the benefits of using Lenti-X GoStix Plus to measure lentiviral titer?
      • What are Lenti-X Packaging Single Shots(VSV-G)?
      • How is IFU/ml calculagted from the GoStix Value (GV, ng/ml p24)
      • What is the experiment to associate GV with functional titer?
      • What is the experiment to associate GV with functional titer?
      • What is the experiment to associate GV with functional titer?
      • What is the experiment to associate GV with functional titer?
      • What is the experiment to associate GV with functional titer?
      • What is the cloning/packaging capacity of an HIV-1-based lentiviral vector?
      • Which types of lentiviral vectors are compatible with Lenti-X Packaging Single Shots?
      • How consistent are packaging results by Lenti-X packaging Single Shots?
      • What type of cells should be used for packaging?
      • Is it possible to use the GoStix Plus app in languages other than English?
      • How do I locate result data downloaded to my mobile device?
      • Is GoStix data saved in the app?
      • How do I know if my titer is outside the range of the GoStix?
      • Does my mobile device need to be connected to the internet?
      • Why do I have to enter the lot number of my kit into the app and where is the lot number found?
      • Why do I NOT need to create a standard curve for Lenti-X GoStix Plus?
      • Can Lenti-X GoStix Plus be used even in the absence of a smartphone?
      • What happens if I do not wait 10 minutes for the signal to develop?
      • How can harvest time affect the GoStix Value?
      • What factors can impact a GoStix titer?
      • How do I correlate GV to IFU/ml or ng p24?
      • Which devices are supported by the GoStix Plus app?
      • Where do I download the GoStix app?
    • What are the main features of adeno-associated viruses?
    • I want to purify my AAV vector. Which kit do you recommend?
    • What are the advantages of the AAVpro Helper Free System in comparison with AAV production kits from other companies?
    • Do you offer AAV control vectors?
    • Infection of cultured cells cannot be confirmed; what could be the cause?
    • What cells are suitable for the production of AAV particles?
    • What is “helper-free” production?
    • How much AAV should be used for general animal experiments?
    • What precautions should be taken when working with recombinant AAV?
    • What are the features of different AAV serotypes?
    • What features of AAV makes the virus a good vector for gene delivery?
    • What is IFU?
    • What is MOI?
  • Retronectin18 Articles

    • How should cells be dissociated from RetroNectin-coated plates?
    • After transduction of cells using RetroNectin reagent, what method do you recommend to resuspend the cells?
    • Is there a way to improve transduction efficiency?
    • Can cell-culture bags (e.g., X-fold bags) be coated with RetroNectin reagent?
    • Does Takara Bio provide a clinical-grade RetroNectin reagent?
    • Does RetroNectin reagent have to be filter sterilized?
    • For how long are pre-coated RetroNectin plates stable?
    • Can RetroNectin reagent be coated on plates that are also coated with collagen?
    • What type of tissue culture plate can be coated with RetroNectin reagent?
    • Can RetroNectin reagent be used in combination with Polybrene?
    • How much cytotoxicity is typically observed when using RetroNectin reagent?
    • Does RetroNectin reagent work for retroviruses packaged with different envelopes?
    • What types of viral vectors can be used with RetroNectin reagent?
    • What cell types can RetroNectin reagent be used with?
    • What are Very Late Antigen-4 (VLA-4) and Very Late Antigen-5 (VLA-5)?
    • How does RetroNectin reagent colocalize viral particles and cells? Where do the virus particles bind?
    • What is Fibronectin?
    • What is RetroNectin reagent?
  • LymphoOne8 Articles

    • Will there be a GMP-grade version of LymphoONE medium?
    • How does LymphoONE medium compare to Takara Bio's previous T-cell medium, GT-T551?
    • Is LymphoONE medium chemically defined?
    • Does LymphoONE medium contain any human-derived components?
    • What other components does LymphoONE medium contain?
    • Does LymphoONE medium contain any animal-derived components?
    • Does LymphoONE medium contain antibiotics?
    • How does LymphoONE medium perform with the addition of serum?
  • Macherey Nagel19 Articles

    • What are the dimensions of 740488?
    • Can I use the NucleoProtect reagent for collecting nasal swabs?
    • Can I purchase RCU buffer from 740910 NucleoSpin RNA Clean-up Maxi?
    • Do you have any special recommendations for isolating viral RNA from breast milk?
    • Is the RLT buffer from Qiagen compatible with NucleoMag Virus? Customer is isolating Covid-19 virus from various samples stored in the RLT buffer.
    • What kit do you recommend for isolating RNA from OCT-fixed tissue blocks?
    • Can I store samples in the RA1 lysis buffer?
    • What is Marcherey Nagel Carrier RNA made of?
    • What are the sizes of holes of the NucleoBond Smart Rack?
    • What are the differences between 744950 (KingFisher 96 Accessory Kit A) and 744951 (KingFisher 96 Accessory Kit)?
    • Can samples lysed in TriZol be used on the NucleoSpin® RNA Set for NucleoZOL™?
    • How do I select NucleoMag® Beads for my specific application?
    • Can the NucleoSpin Gel & PCR Clean-up kit be used for removing the TB Green Dye?
    • What are the dimensions of the NucleoSpin® 96 Plasmid Filter Plates?
    • Is it OK to freeze cells in the MR1+TCEP at -80 C? I couldn't find freezing recommendations in the user manual for the NucleoMag RNA kit.
    • Is NucleoZOl compatible with NucleoMag kits (Pathogen)?
    • Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
    • What are the dimensions of the NucleoVac™ 96 Vacuum Manifold?
    • Can the NucleoSpin Plasmid Purification columns substitute the NucleoSpin Plasmid Transfection- grade columns to isolate Endo-free plasmid DNA?
  • New Citations since 20230 Articles

    • Bakhyt citations1 Articles
      • A new deep-sea sponge Fibulia occiensis sp. nov. (Poecilosclerida: Dendoricellidae) from the 25° South Oceanic Core Complex in the Central Indian Ocean Ridge
    • Ian citations1 Articles
      • Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery
  • Spacial Genomics0 Articles

    • Seeker35 Articles
      • Is Curio Seeker compatible with Element Biosciences Aviti Sequencer?
      • What is the recommended number reads when your sample tissue does not cover the entire Tile area?
      • How much PhiX should I spike in to my library before sequencing?
      • What are the PhiX recommendations for the NovaSeq X Plus?
      • How deep should I sequence Curio Seeker libraries?
      • For the Dual Indexing Primer kit, what orientation are the sequences listed in the User Guide table?
      • Where can I find the bam file where reads are associated with bead barcodes and/or molecular barcodes?
      • What do I do if the pipeline says it cannot find my STAR reference when it clearly exists?
      • How do I fix the following error message? Remote resource not found: https://api.github.com/repos/nextflow-io/commercial_seeker/contents/main.nf
      • What do I do if the FORMATCONVERT step fails with the following error?
      • CURIOSEEKER_GEN_GENE_BARCODE_UMI_DB step failed with the following error. How do I resolve it?
      • If I have multiple regions or tissues of interest on a tile, how do I select only the beads for the region I am interested in?
      • How do you get untrimmed reads from the sequencer?
      • What method of clustering do we use for the UMAP?
      • What is the conversion of coordinate system to um?
      • Do you have any guidance on trimming FASTQ files to 2 x 50?
      • What are the minimum system requirements for local installation of the Curio primary analysis pipeline?
      • How do I recalculate "median reads/UMI" after filtering out beads not covered by tissue?
      • How do I filter out the background in the sample?
      • What if my library has indications of PCR-bubble formation?
      • What is difference between the Dual Indexing primer kit V1 and V2?
      • Do you need to optimize the permeabilization step for Curio Seeker?
      • If I have low cDNA concentration, how do I re-amplify it?
      • Do I need to make any adjustments for running Human Heart tissue?
      • What modifications do I need to make for running Curio Seeker on plant tissue?
      • How do I harvest and preserve fresh frozen tissue for sectioning?
      • Is the use of the CryoCube essential? What if it is not used?
      • How thick should my tissue sections be for the Curio Seeker tile?
      • How do we use the CryoCube?
      • What kind of tissue and sample types can I use with the Curio Seeker kit?
      • Does Curio Seeker require any specialized hardware or instrumentation?
      • How large is the Curio Seeker tile?
      • What is the spatial resolution of the Curio Seeker tile?
      • How long does the Curio Seeker workflow take?
      • What is the Curio Seeker kit?

Popular Knowledgebase Articles

  • FAQ
    • 61
    • 0
    • 0
  • Customer’s stool samples were collected in ithe Genotek Omnigen collection tubes (OMR-200). What are the recommendations for Genotek Omnigen and NucleoSpin® DNA Stool kit?
    • 380
    • 0
  • What is the diameter of the NucleoMag Magnetic bead particles? 
    • 324
    • 0
  • Are NucleoMag Beads Silica-based?
    • 292
    • 0

Company Sites

Home

New products

Special offers

Instruments & reagent services

Contact Us

Technical support

Customer service

Sales

Shipping & delivery

Categories

  • Nucleic Acid Purification (Macherey Nagel)
  • NGS
  • PCR
  • In-Fusion
  • qPCR
  • -- All Categories --

Connect With Us

  • Takara Bio USA support portal
  • Biotechnology reagent provider
  • 2560 Orchard Parkway
    San Jose, CA 95110


    Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

  • 800.662.2566
  • [email protected]
  • https://www.takarabio.com/
    Powered by LiveHelpNow customer support suite