Can I use In-Fusion Cloning to clone a DNA fragment generated by restriction digest?
Can I use In-Fusion Cloning to clone a DNA fragment generated by restriction digest?
Yes—if the adjacent DNA fragments/oligos or linearized vector carry the 15-bp homologous overlaps required for annealing. 15-bp overlaps with the digested cloning insert may be added to the termini of a PCR-linearized vector or a synthetic oligonucleotide.