How do I correlate GV to IFU/ml or ng p24?
How do I correlate GV to IFU/ml or ng p24?
Lenti-X GoStix Plus-based titers can be used in a similar fashion to ELISA-based titers. In the example of Lenti-X ZsGreen1 lentivirus, the virus stock was prepared using the Lenti-X Packaging Single Shots lentiviral packaging system. Percentages of cells transduced (% transduced) were determined on HT1080 cells via FACS analysis. In addition, levels of lentiviral p24 from this virus stock were assessed using the Lenti-X p24 Rapid Titer Kit (ELISA) and Lenti-X GoStix Plus. Transductions were performed on HT1080 cells using a range of MOIs and the resulting IFU/ml can be calculated from the fluorescence. This functional IFU/ml was calculated against the associated ELISA and GoStix Values (ng/ml p24) to obtain conversion factors of GV and IFU/ml.