Takara enzyme for bisulfite-treated DNA
Takara enzyme for bisulfite-treated DNA
Several choices are available:
1. TaKaRa EpiTaq HS (for bisulfite-treated DNA) is optimized for PCR amplification using bisulfite-treated DNA templates that contain uracil. This enzyme includes a hot-start antibody and is designed for use during methylation analysis, including COBRA and bisulfite-sequencing analyses.
2. The EpiScope MSP Kit is designed specifically for methylation-specific PCR (MSP) assays.
3. TaKaRa Taq DNA Polymerase and TaKaRa Taq DNA Polymerase Hot Start Version, both of which lack 3'?5' exonuclease activity, may be used in some instances.