How frequently should I passage cells grown in DEF-CS
Most human iPS cell lines can be passaged every 3-4 days. We suggest the following weekly schedule: passage on Monday and Thursday, using a slightly higher seeding density on Monday, then change the media on Tuesday, Wednesday, Friday, and Sunday. We recommend seeding 4.0-5.0 x 104 cells/cm2and passaging at a density of 1.5-3.0 x 105 cells/cm2.
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