Can I use TOP10 cells for In-Fusion Cloning?
Can I use TOP10 cells for In-Fusion Cloning?
TOP10 cells or their derivatives (e.g., ccdB Survival 2T1R E. coli), and related strains (e.g., DH10B, MC1061) are suboptimal for In?Fusion Cloning, resulting in a lower number of recombinant clones. This may be of particular concern if you are performing multiple-fragment cloning, or using a low-copy number vector.
We recommend using Stellar Competent Cells, which are optimized for use with In Fusion Cloning and are included in all current kits.