What cells are suitable for the production of AAV particles?
What cells are suitable for the production of AAV particles?
For the AAVpro Helper Free Systems, use HEK 293T or HEK 293 cells. Several HEK 293 and HEK 293T cell lines are commercially available. Viral production is highly dependent on features of the cell line. The AAVpro 293T Cell Line* or HEK 293T/17 cells (ATCC, CRL-11268) are recommended for preparation of high-titer AAV.
The AAVpro 293T Cell Line is a subclone of the transformed human embryonic kidney cell line HEK 293, which is highly transfectable and supports high levels of viral protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. When combined with the AAVpro Helper Free System, these cells can produce high titers of recombinant adeno-associated virus (AAV).