What insert sources are compatible with In-Fusion?
What insert sources are compatible with In-Fusion?
The following types of inserts are compatible with In?Fusion Cloning:
-PCR-amplified DNA fragments carrying overhangs complementary with the termini of the adjacent DNA fragment(s), synthetic oligos, or linear vector.
-DNA fragments generated by restriction digest with one or more restriction enzymes. In this instance, the required 15-bp homology must be carried by the adjacent DNA fragment(s), synthetic oligos, or linear vector.
-Synthetic oligonucleotides (?50 bp) with 15-bp homology to the termini of adjacent fragments or the linearized vector. High-quality, non-phosphorylated oligonucleotides purified by desalting are compatible with In?Fusion Cloning. Gel or HPLC purification of oligonucleotides is not required.