What are the features of Takara online primer design tools?
What are the features of Takara online primer design tools?
Instructions for designing In?Fusion PCR primers are included in all In?Fusion Cloning user manuals. Several tools are also available to help with the process.
1. Our online Primer Design Tool facilitates primer design for In?Fusion Cloning, and is compatible with Mozilla Firefox or Google Chrome web browsers. (Internet Explorer is not compatible with the Primer Design Tool.) Specific cloning conditions supported are as follows:
-Single-fragment cloning
-Multiple-fragment cloning
-Vectors linearized by restriction digest
-Vectors linearized by inverse PCR
-Prelinearized vectors
-Insert-specific primers designed by the user
-Insert-specific primers generated by the Primer Design Tool
2. The current version of the Primer Design Tool does not allow adjustment for translational reading frame continuity, which should be manually designed by the user.
3. We also recommend SnapGene Viewer as a helpful, free online tool for in silico assembly of your recombinant construct, manual design of In?Fusion PCR primers, and adjustment of translational reading frame continuity.