What type of tissue culture plate can be coated with RetroNectin reagent?
What type of tissue culture plate can be coated with RetroNectin reagent?
Non-coated tissue culture plates or dishes should be used. Takara Bio R&D scientists have tested both coated and non-coated plates and found that RetroNectin coating efficiency is reduced by 1/5 for coated plates. Experimental data indicate that transduction efficiency is above 30% when using RetroNectin-coated, non-tissue culture treated plates, but only 1–3% when using RetroNectin-coated tissue culture-treated plates. Six-well BD-Falcon cell-culture plates (Cat. # 351146) are recommended.