What is “helper-free” production?
What is “helper-free” production?
In general, replication of AAV requires a helper virus such as adenovirus or herpesvirus. Our AAV helper-free vector systems generate recombinant AAV without the use of a helper virus ("helper-free"). Instead, packaging is accomplished by transfecting HEK 293 cells (e.g. AAVpro 293T Cell Line) with plasmids that encode the factors necessary to prepare recombinant AAV particles.
