PrimeSTAR Max and PrimeSTAR GXL DNA polymerases have very high fidelity; how was fidelity measured for these enzymes?
PrimeSTAR Max and PrimeSTAR GXL DNA polymerases have very high fidelity; how was fidelity measured for these enzymes?
The fidelities of the PrimeSTAR polymerases were measured by Sanger sequencing of individual colonies after PCR, as described below:
-Ten arbitrarily selected GC-rich regions of Thermus thermophilus HB8 genomic DNA were amplified.
-PCR products were cloned into a plasmid vector.
-Multiple clones were selected for each respective amplification product, and the PCR insert was sequenced.
PrimeSTAR Max DNA Polymerase has a fidelity 29X that of Taq polymerase, whereas PrimeSTAR GXL DNA Polymerase has a fidelity 6.5X that of Taq polymerase.