How do I split the 15-nt homologous overlap among adjacent DNA fragments?

related articles

• How does In-Fusion Snap Assembly compare to NEBuilder HiFi DNA Assembly?
  • 149
  • 0
• How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?
  • 102
  • 0
• What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?
  • 68
  • 0
• What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?
  • 96
  • 0
• How do I calculate the 15-nt overlap if the vector is linearized via restriction digest, generating a 5' or 3' overhang?
  • 94
  • 0
• How do I clone my gene of interest in the same translational reading frame as a tag present in the cloning vector (e.g., fluorescent protein, Myc, HA, etc.)?
  • 91
  • 0
• Can small external sequences be included in the In-Fusion PCR primer?
  • 76
  • 0
• For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?
  • 111
  • 0
• What oligonucleotide quality is required for an In-Fusion PCR primer?
  • 68
  • 0
• Can I clone multiple fragments into one vector in a single In-Fusion Cloning reaction?
  • 74
  • 0