What is “helper-free” AAV production?
In general, replication of AAV requires a helper virus such as adenovirus or herpesvirus. Our AAV helper-free vector systems generate recombinant AAV without the use of a helper virus ("helper-free"). Instead, packaging is accomplished by transfecting HEK 293 cells (e.g. AAVpro 293T Cell Line) with plasmids that encode the factors necessary to prepare recombinant AAV particles.
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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.