How do I preserve restriction-site using In-Fusion?
How do I preserve restriction-site using In-Fusion?
Primer design lets you easily preserve or eliminate the restriction sites used to linearize the cloning vector. In order to maintain restriction sites at cloning junctions, nucleotides can be added to the PCR primers between the template-specific portion and the 15-nt homologous overlap.
The online Primer Design Tool offers the option to preserve the restriction site(s) (the Primer Design Tool is compatible with Mozilla Firefox and Google Chrome web browsers, but not with Internet Explorer).