How does LymphoONE medium perform with the addition of serum?
How does LymphoONE medium perform with the addition of serum?
While LymphoONE medium was developed to enable efficient T-cell expansion in the absence of serum, researchers always have the option of supplementing the medium with serum or plasma in order to achieve greater proliferation. As demonstrated below by the data involving stimulation by IL-2, anti-CD3 mAb, and RetroNectin reagent, supplementation of LymphoONE medium with human serum results in greater T-cell expansion and yields higher proportions of naïve T cells (CD45RA+/CCR7+ phenotype) compared to serum-free conditions. The data also demonstrate that LymphoONE medium outperforms competitor media under both scenarios (with or without addition of serum).