For administration to animals, how should AAV particles be purified?
For administration to animals, how should AAV particles be purified?
There are no definitive purity criteria for in vivo transduction. You can confirm AAV purity by SDS-PAGE analysis; there should only be three major bands (corresponding to AAV VP1, VP2, and VP3).
AAV particles purified using the AAVpro Purification Kit (All Serotypes) have been used successfully for in vivo transduction in several animal species.