What is a GoStix Value (GV) titer?
What is a GoStix Value (GV) titer?
The GoStix Plus app scans the GoStix cassette for the test (T) and control (C) bands, measures the intensity of each band, and then performs a calculation that generates a metric which we refer to as the GoStix Value (GV; equivalent to ng/ml p24). The intensity of the test band on the cassette increases with the amount of p24 protein in your lentiviral supernatant, which in turn correlates with increasing infectious units (IFU). The GV is correlated to infectious titer in the same way that ng p24 or genome copies (gc) correlate to infectious titer when using traditional p24 ELISAs or qRT-PCR assays, respectively, to determine titer. As is the case with those other assays, the GV that is best for each experimental application will vary depending on several factors including the target cells, the MOI required, and the lentiviral vector and packaging system used.