Can I transform In-Fusion Cloning reaction mixtures in amounts larger than what is recommended in the user manual?
Can I transform In-Fusion Cloning reaction mixtures in amounts larger than what is recommended in the user manual?
We do not recommend this. Transforming the reaction mixture in an amount larger than what is stated in the user manual may be toxic to your cells. For transformation of the In-Fusion Cloning reaction, use 2.5 µl of undiluted, unpurified reaction mix per 50 µl of Stellar Competent Cells.
(Optional) For larger transformation volumes, 5.0 µl of undiluted, unpurified reaction mix can be transformed per 100 µl of Stellar Comptent Cells.