What are the characteristics of human iPS cells cultured with the DEF-CS system?
What are the characteristics of human iPS cells cultured with the DEF-CS system?
Human iPS cells cultured with the DEF-CS system are typically 98% Oct-4 positive, as determined by flow cytometry. They also express common pluripotency markers, including the transcription factors Oct-4 and NANOG and the surface markers SSEA-4, TRA-1-60, and TRA-1-81. The cells do not express the differentiation marker SSEA-1. Cells grown in the DEF-CS system have been shown to differentiate in vitro and in vivo into all three germ layers.