What RNA quality is needed for performing full length Next Generation Sequencing (NGS)?

RNA quality is an important consideration when performing full length Next Generation Sequencing (NGS) Key factors affecting the quality of RNA include the integrity of the sample and any contamination that may have occurred during collection or processing. Poor RNA quality can lead to missing data and inaccurate results, whereas high quality RNA can provide more comprehensive and precise results. To ensure RNA quality is suitable for full length NGS sequencing, it is important to assess integrity, purity and mRNA concentration. It is also necessary to check for any contaminants that may have been introduced during collection or processing. Factors which may indicate poor quality RNA include increased fragmentation, contamination with proteins or nucleases, and inconsistencies in cDNA size. To accurately analyze the quality of an RNA sample, it is recommended to utilize quantitative assays, such as capillary electrophoresis. These assays allow for comparison of the RNA sample to a known reference to determine integrity and assess contaminants. Additionally, spectrophotometric readings may be taken to measure the amount of RNA present in the sample. With these quantitative methods, laboratories can make informed decisions regarding the suitability of an RNA sample for full length NGS sequencing.