Can I modify translational reading frames during In-Fusion?
Can I modify translational reading frames during In-Fusion?
In-Fusion Cloning makes it possible to seamlessly clone your gene of interest in the same translational reading frame as a desired tag (e.g., fluorescent protein, Myc, HA, etc.). Specifics in the design of In-Fusion PCR primers facilitate this application, allowing you to maintain reading frame continuity in the recombinant vector. Two options for doing so are below:
-Adjusting the length of the template-specific (gene-specific) portion of the In?Fusion PCR primer
-Adding nucleotides between the template-specific portion and the 15-nt homologous overlap portion of the In?Fusion PCR primer
Please note that the current version of our online Primer Design Tool does not allow adjustment for translational reading frame continuity. The primer sequence should be manually designed by the user. We recommend SnapGene Viewer as a helpful, free online tool to help with this task.