Does fetal CYP3A7 contribute to overall CYP activity in these cells?
Does fetal CYP3A7 contribute to overall CYP activity in these cells?
Whereas a CYP activity assay cannot discriminate between CYP3A4 (adult), CYP3A5 (adult), and CYP3A7 (fetal), qPCR can. In our tests, Cellartis enhanced hiPS-HEP cells expressed high mRNA levels of CYP3A4 and CYP3A5 and very low levels of CYP3A7—comparable to cryopreserved human primary hepatocytes cultured for 24 hours (Panel A). Interestingly, interindividual variation in CYP expression can be observed in Cellartis enhanced hiPS-HEP cells derived from different hiPS cell lines. In Panel B, mRNA expression of CYP enzymes in human primary hepatocytes is shown as a dashed line.
