Can I purify adenovirus from cell supernatants or cells combined with supernatant using Adeno-X purification kits?
Can I purify adenovirus from cell supernatants or cells combined with supernatant using Adeno-X purification kits?
No. The Adeno-X Maxi/Mega purification kits only purify virus from cell pellets, which accumulate most of the amplified virus, provided that the cells are harvested prior to complete cell lysis (Figure 1). This protocol greatly simplifies adenoviral purification.