Do Cellartis enhanced hiPS-HEP cells express transporter proteins?
Do Cellartis enhanced hiPS-HEP cells express transporter proteins?
Yes. In our tests, Cellartis enhanced hiPS-HEP cells derived from ChiPSC12, ChiPSC18, and ChiPSC22 expressed several transporters: NTCP, OCT1, OATP1B1, and OATP1B3 (uptake, Panel A); plus MRP2, MDR1, and BSEP (efflux, Panel B). Some transporters were expressed at similar levels as cryopreserved human primary hepatocytes (hphep); other transporters were expressed at lower levels than hphep. mRNA expression of OATP1B3 was not observed in enhanced hiPS-HEP cells derived from ChiPSC18. In Panel C, mRNA expression from hphep is shown as a dashed line.
