Which retroviral packaging cell line or system should I use to transduce my target cells?
Which retroviral packaging cell line or system should I use to transduce my target cells?
Viral envelopes are classified by the receptors they use to enter host cells. Your virus' range of infectivity (cell tropism) is determined by its envelope, which is in turn conferred by the packaging cell line or system. You should choose your packaging cell line or system based on your target cells.
EcoPack 2-293 Cell Line (HEK 293), Tropism: Ecotropic, Envelope: gp70, Receptors: mCAT1, gag-pol: Bleo, Selection: Hygro, Host cell: Rat and mouse
AmphoPack 293 Cell Line (HEK 293), Tropism: Amphotropic, Envelope: 4070A, Receptors: Ram-1 (rPit-2), gag-pol: Bleo, Selection: Puro, Host cell: Many mammalian cell types
RetroPack PT67 Cell Line (NIH 3T3), Tropism: Dualtropic, Envelope: 10A1, Receptors: GALV, RAM, gag-pol: TK, Selection: DHFR, Host cell: Many mammalian cell types
GP2-293 Cell Line (HEK 293), Tropism/Envelope/Receptors: depends on the envelope proteins transfected, gag-pol: DHFR, Host cell: All cell types