When should library quantification be performed for ThruPLEX DNA-Seq Kit?
When should library quantification be performed for ThruPLEX DNA-Seq Kit?
Quantification must be performed prior to sequencing. It is normally done after the AMPure XP purification step, but libraries can also be quantified immediately following the Library Amplification Reaction to normalize samples prior to pooling. If yield is of concern, the quantification method used must not require purification of the library. Please refer to the ThruPLEX DNA-Seq Kit User Manual for the library processing workflow after library preparation and for instructions on library quantification.