What are the optimal cell density and passage number for transfection?
What are the optimal cell density and passage number for transfection?
The overall health of target cells at the time of transfection is a key factor for transfection efficiency. We recommend using a 60–75% confluent culture for plasmid transfection—this is when cells are optimally active, and the risk of reagent toxicity is low. Xfect products are highly efficient with little to no cytotoxicity, further increasing your chances of success.Cell culture quality decreases with repeated passages, so keep the cell passage number low. Passage newly thawed cells at least twice and plate prior to transfection as instructed in the reagent's user manual.