Does In-Fusion Cloning preserve the restriction site(s) used to linearize the vector?
Does In-Fusion Cloning preserve the restriction site(s) used to linearize the vector?
In order to maintain the restriction sites, nucleotides can be added to the PCR primers between the template-specific portion and the 15-nt homologous overlap.
The online Primer Design Tool allows you to choose whether or not to preserve the restriction sites. (The Primer Design Tool is compatible with Mozilla Firefox or Google Chrome web browsers, but not with Internet Explorer.)