What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?
What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?
Instructions for designing In?Fusion PCR primers are included in all In?Fusion Cloning user manuals. Additionally, our online Primer Design Tool facilitates primer design for single- and multiple-fragment cloning and is compatible with Mozilla Firefox or Google Chrome web browsers (Internet Explorer is not compatible with the Primer Design Tool).
We also recommend SnapGene Viewer as a helpful, free online tool for in silico assembly of your recombinant construct, the manual design of In?Fusion PCR primers, and adjustment of translational reading frame continuity.