What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?

related articles

• What is the difference between In-Fusion Snap Assembly and In-Fusion Snap Assembly EcoDry?
  • 69
  • 0
• What is Cloning Enhancer (CE)?
  • 44
  • 0
• Does the In-Fusion Cloning method introduce errors into the sequence?
  • 44
  • 0
• What is the difference between In-Fusion Snap Assembly and In-Fusion HD Cloning?
  • 88
  • 0
• In-Fusion HD and In-Fusion Snap Assembly bundles include different PCR polymerases. Should I expect a difference in performance?
  • 43
  • 0
• How does In-Fusion Snap Assembly compare to NEBuilder HiFi DNA Assembly?
  • 79
  • 0
• How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?
  • 55
  • 0
• How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?
  • 47
  • 0
• What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?
  • 38
  • 0
• What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?
  • 40
  • 0