What is the appropriate number of replicates (n)?
What is the appropriate number of replicates (n)?
The necessary number of sample replicates (n) varies depending on the experimental system. In principle, when the experimental error is expected to be relatively large, use a larger number of samples.
*When profiling gene expression by RT-qPCR, it is useful to prepare multiple biological replicates and perform multiple RNA extractions in order to ascertain the degree of biological variability.
*With respect to qPCR, greater variability is expected when using a low level of template because the number of cycles required for detection will be high. In such cases, use a larger number of replicates.