What are the advantages of using an sgRNA library in Takara Bio’s Lenti-X Single Shots format vs. starting from plasmid or lentiviral particles?
What are the advantages of using an sgRNA library in Takara Bio’s Lenti-X Single Shots format vs. starting from plasmid or lentiviral particles?
Researchers conducting pooled, lentiviral CRISPR knockout screens often start either with an sgRNA library in plasmid form or premade lentiviral particles. Starting from plasmid involves a much lower upfront cost, but the process of amplifying the library, verifying sgRNA representation, and producing lentivirus require familiarity with a wide array of techniques and often proves to be time-consuming and overly challenging for some researchers. Starting from lentiviral particles allows researchers to circumvent the challenges of library amplification and validation and production of lentivirus but is exceedingly expensive.