Can TALON columns/resin be regenerated?
Can TALON columns/resin be regenerated?
Yes, TALON columns can be regenerated by washing with a MES/NaCl buffer to remove the imidazole. However, it cannot be guaranteed that the binding capacity after regeneration will be the same as that of the original unused resin. Takara Bio recommends that a regenerated column only be used to purify the same protein that it was previously used to purify, to prevent any contamination. Follow the instructions for regenerating TALON resin in the TALON Metal Affinity Resins User Manual.