Is the performance of TALON resin affected by the temperature?
Is the performance of TALON resin affected by the temperature?
If the protein requires colder temperatures to prevent degradation, the purification steps can be performed at 4°C. This is a matter of preference, as the resin will work at either room temperature or 4°C. However, the resin should never be allowed to freeze because the agarose beads can break, leaking metal ions and decreasing binding capacity.