What is the Brunello algorithm that was used to create the library?
What is the Brunello algorithm that was used to create the library?
The target sequences cloned into the Guide-it Genome-Wide sgRNA Library are the same as described by Doench et al. in 2016. In this paper, the authors created and modified their algorithm based on real screening and activity data from positive and negative screens. They named their human library ""Brunello.""
ReferenceDoench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR Cas9. Nat. Biotechnol. 34, 184–191 (2016).