What total amount of DNA is needed for Covaris shearing, and what type of buffer volumes are used during the shearing?
What total amount of DNA is needed for Covaris shearing, and what type of buffer volumes are used during the shearing?
Successful shearing has been accomplished with 2–50 ng of DNA in 50–130 µl of TE or low-TE buffer. If you are working with smaller amounts of DNA, see the ""Low-volume DNA shearing for ThruPLEX library prep"" tech note.