What read length is recommended for the SMARTer Human TCR-v2 libraries?
What read length is recommended for the SMARTer Human TCR-v2 libraries?
For read through the entire V(D)J reagion use 600-cycle MiSeq Reagent Kit v3 with 2 x 300 base pair reads. To cover the entire CDR3 region and UMI use 2 x 150 base paired-end reads, following Illumina instrument and kit combinations provided in the the SMARTer Human TCR a/b Profiling Kit v2 User Manual - Table 5. Illumina instrument and reagent kit recommendations.