Does SMARTer Human BCR IgG IgM H/K/L Profiling Kit require rRNA depletion prior to cDNA synthesis?
Does SMARTer Human BCR IgG IgM H/K/L Profiling Kit require rRNA depletion prior to cDNA synthesis?
No, SMARTer Human BCR IgG IgM H/K/L Profiling Kit utilizes oligo(dT) priming for the first-strand cDNA synthesis (annealing to poly(A) mRNAs) followed by BCR-specific amplification, thus generating BCR-specific cDNA. It is advisable to use high integrity (RIN>7) input RNA for the SMARTer Human BCR IgG IgM H/K/L Profiling Kit.