What read length is recommended for the SMARTer® Human BCR IgG IgM H/K/L libraries?
What read length is recommended for the SMARTer® Human BCR IgG IgM H/K/L libraries?
For read through the entire V(D)J reagion use 600-cycle MiSeq Reagent Kit v3 with 2 x 300 base pair reads. To cover the entire CDR3 region and UMI use Read1-200 bp and Read2- 50bp.