What is the expected size range of Covaris-sheared double-stranded (ds) cDNA?
What is the expected size range of Covaris-sheared double-stranded (ds) cDNA?
Covaris-sheared ds cDNA should span 100–500 bp with a peak of approximately 200 bp. To ensure optimal Covaris ds cDNA shearing:
Do not load more than 75 µl of ds cDNA per 100 µl Covaris tube.
Avoid introducing air bubbles when loading the ds cDNA in the Covaris tube.
Example Bioanalyzer electropherogram of Covaris-sheared ds cDNA. Recommendations for Covaris DNA shearing conditions can be found in SMARTer Ultra low kit user manuals.